Processo De Obtenção De Poliésteres Polinitrosados Como Doadores Poliméricos De óxido Nìtrico Para Aplicações Biomédicas

Novo processo ou método de síntese de um novo material polimérico, com estrutura de um poliéster polinitrosado com grupos funcionais S-NO, a partir da reação de poliesterificação de glicóis com ácidos carbixílicos. O novo material e capaz de atuar como doador espontâneo de NO por via térmica ou fotoquímica. O presente invento se refere aos processos de síntese de uma nova classe de materiais, e dos materiais propriamente ditos, que consistem em poliésteres polinitrosados, formados a partir da reação de poliesterificação de um glicol com um acido dicarboxilico que contenha em sua estrutura grupos tióis (-SH), obtendo-se um poliéster polisulfidrilado, seguida da reação de S-nitrosacão dos grupos tióis deste poliéster, que são então convertidos em grupos S-nitrosotióis (-SNO), doadores de NO. Sendo estes materiais utilizados preferencialmente em aplicações biomédicas.BR0300784 (A)C08G63/78C08G63/78BR20030300784C08G63/78C08G63/7

Droplet microfluidics for the highly controlled synthesis of branched gold nanoparticles

The synthesis of anisotropic metallic nanoparticles (NPs) has been a field of intense and challenging research in the past decade. In this communication, we report on the reproducible and highly controllable synthesis of monodisperse branched gold nanoparticles in a droplet-based microfluidics platform. The process has been automated by adapting two different bulk synthetic strategies to microdroplets, acting as microreactors, for NP synthesis: a surfactant-free synthesis and a surfactant-assisted synthesis. Microdroplets were generated in two different microfluidic devices designed to accommodate the requirements of both bulk syntheses. The epitaxial growth of AuNSTs inside the microdroplets allowed for a fine control of reagent mixing and local concentrations during particle formation. This is the first time branched gold NPs have been synthesised in a microfluidics platform. The monodispersity of the product was comparable to the synthesis in bulk, proving the potential of this technology for the continuous synthesis of high quality anisotropic NPs with improved reproducibility.This work was supported by EU Framework Programme for Research and Innovation H2020 COFUND, Grant Agreement 713640 (SAC) and by FAPESP, project No. 2015/01685-2 (PTB). MGO acknowledges FAPESP, project No 2016/02414-5

Droplet microfuidics for the highly controlled synthesis of branched gold nanoparticles

The synthesis of anisotropic metallic nanoparticles (NPs) has been a feld of intense and challenging research in the past decade. In this communication, we report on the reproducible and highly controllable synthesis of monodisperse branched gold nanoparticles in a droplet-based microfuidics platform. The process has been automated by adapting two diferent bulk synthetic strategies to microdroplets, acting as microreactors, for NP synthesis: a surfactant-free synthesis and a surfactantassisted synthesis. Microdroplets were generated in two diferent microfuidic devices designed to accommodate the requirements of both bulk syntheses. The epitaxial growth of AuNSTs inside the microdroplets allowed for a fne control of reagent mixing and local concentrations during particle formation. This is the frst time branched gold NPs have been synthesised in a microfuidics platform. The monodispersity of the product was comparable to the synthesis in bulk, proving the potential of this technology for the continuous synthesis of high quality anisotropic NPs with improved reproducibility.8FAPESP – Fundação de Amparo à Pesquisa Do Estado De São Paulo2015/01685-2; 2016/02414-

Avaliação da toxicidade na superfície ocular após instilação tópica de doadores de óxido nítrico

PURPOSE: To evaluate the ocular surface toxicity of two nitric oxide donors in ex vivo and in vivo animal models: S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC) in a hydroxypropyl methylcellulose (HPMC) matrix at final concentrations 1.0 and 10.0 mM. METHODS: Ex vivo GSNO and SNAC toxicities were clinically and histologically analyzed using freshly excised pig eyeballs. In vivo experiments were performed with 20 albino rabbits which were randomized into 4 groups (5 animals each): Groups 1 and 2 received instillations of 150 µL of aqueous HPMC solution containing GSNO 1.0 and 10.0 mM, respectively, in one of the eyes; Groups 3 and 4 received instillations of 150 µL of aqueous HPMC solution-containing SNAC 1.0 and 10.0 mM, respectively, in one of the eyes. The contralateral eyes in each group received aqueous HPMC as a control. All animals underwent clinical evaluation on a slit lamp and the eyes were scored according to a modified Draize eye test and were histologically analyzed. RESULTS: Pig eyeballs showed no signs of perforation, erosion, corneal opacity or other gross damage. These findings were confirmed by histological analysis. There was no difference between control and treated rabbit eyes according to the Draize eye test score in all groups (p>0.05). All formulations showed a mean score under 1 and were classified as non-irritating. There was no evidence of tissue toxicity in the histological analysis in all animals. CONCLUSION: Aqueous HPMC solutions containing GSNO and SNAC at concentrations up to 10.0 mM do not induce ocular irritation.OBJETIVO: Avaliar a toxidade na superfície ocular de dois compostos doadores de óxido nítrico em modelos ex vivo e in vivo: S-nitrosoglutationa (GSNO) e S-nitroso-N-acetilcisteína (SNAC), em uma matriz de hidroxipropil metilcelulose (HPMC) nas concentrações finais de 1,0 and 10,0 mM. MÉTODOS: As toxicidades de GSNO e SNAC foram avaliadas clinicamente e histologicamente em modelo ex vivo usando globos oculares porcinos recém excisados. Experimentos in vivo foram realizados com 20 coelhos albinos que foram randomizados em 4 grupos (5 animais em cada): Os grupos 1 e 2 receberam instilações de 150 µL de solução aquosa de HPMC contendo GSNO 1,0 e 10,0 mM, respectivamente, em um dos olhos; Os grupos 3 e 4 receberam instilações de 150 µL de solução aquosa de HPMC contendo SNAC 1,0 and 10,0 mM, respectivamente, em um dos olhos. Os olhos contralaterias em cada grupo receberam solução aquosa de HPMC como controle. Todos os animais foram clinicamente avaliados em lâmpada de fenda e os olhos foram pontuados de acordo com o teste de Draize modificado e analisados histologicamente. RESULTADOS: Os globos oculares porcinos não apresentaram sinais de perfuração, erosão, opacidade da córnea ou outros danos graves. Esses resultados foram confirmados pela análise histológica. Não houve diferença entre os olhos dos coelhos tratados e controles de acordo com a pontuação do teste de Draize em todos os grupos (p>0,05). Todas as formulações apresentaram um escore médio menor do que 1 e foram classificadas como não-irritantes. Não houve evidência de toxicidade tecidual nas análises histológicas em todos os animais. CONCLUSÃO: Soluções aquosas de HPMC contendo GSNO e SNAC em concentrações até 10,0 mM não induzem irritação ocular.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo (UNIFESP) Department of OphthalmologyUniversidade Estadual de Campinas Institute of ChemistryUniversidade Federal de São Paulo (UNIFESP) Department of PathologyUNIFESP, Department of OphthalmologyUNIFESP, Department of PathologySciEL

S-Nitroso-N-Acetylcysteine Ameliorates Ischemia-Reperfusion Injury In The Steatotic Liver

BACKGROUND: Steatosis is currently the most common chronic liver disease and it can aggravate ischemia-reperfusion (IR) lesions. We hypothesized that S-nitroso-N-acetylcysteine (SNAC), an NO donor component, can ameliorate cell damage from IR injury. In this paper, we report the effect of SNAC on liver IR in rats with normal livers compared to those with steatotic livers. METHODS: Thirty-four rats were divided into five groups: I (n=8), IR in normal liver; II (n=8), IR in normal liver with SNAC; III (n=9), IR in steatotic liver; IV (n=9), IR in steatotic liver with SNAC; and V (n=10), SHAN. Liver steatosis was achieved by administration of a protein-free diet. A SNAC solution was infused intraperitoneally for one hour, beginning 30 min. after partial (70%) liver ischemia. The volume of solution infused was 1 ml/100 g body weight. The animals were sacrificed four hours after reperfusion, and the liver and lung were removed for analysis. We assessed hepatic histology, mitochondrial respiration, oxidative stress (MDA), and pulmonary myeloperoxidase. RESULTS: All groups showed significant alterations compared with the group that received SHAN. The results from the steatotic SNAC group revealed a significant improvement in liver mitochondrial respiration and oxidative stress compared to the steatotic group without SNAC. No difference in myeloperoxidase was observed. Histological analysis revealed no difference between the non-steatotic groups. However, the SNAC groups showed less intraparenchymal hemorrhage than groups without SNAC (p=0.02). CONCLUSION: This study suggests that SNAC effectively protects against IR injury in the steatotic liver but not in the normal liver

S-nitrosothiols As Platforms For Topical Nitric Oxide Delivery

Nitric oxide (NO) is a Small radical species involved in several fundamental physiological processes, including the control of vascular tone, the immune response and neuronal signalling. Endothelial dysfunction with the decreased NO bioavailability is the underlying cause of several diseases and has led to the development of a wide range of systemic NO donor compounds to lower the blood pressure and control hypertensive crises. However, several potential therapeutic actions of NO, not related to the cardiovascular system, demand exclusively local actions. Primary S-nitrosothiols (RSNOs) are endogenously found NO carriers and donors and have emerged as platforms for the localized delivery of NO in topical applications. Formulations for this purpose have evolved from low molecular weight RSNOs incorporated in polymeric films, hydrogels and viscous vehicles, to polymeric RSNOs where the SNO moiety is covalently bound to the polymer backbone. The biological actions displayed by these formulations include the increase in dermal vasodilation, the acceleration of wound healing, the killing of infectious microorganisms and an analgesic action against inflammatory pain. This MiniReview focuses on the state of the art of experimental topical formulations for NO delivery based on S-nitrosothiols and their potential therapeutic applications.11934956Annual Meeting of the Scandinavian-Physiological-SocietySep 18-20, 2015Aarhus, DENMAR

Caracterização de oxidos de manganes (IV) suportados em polietileno

Orientador : Fernando GalembeckDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de QuimicaMestrad

Estudo de propriedades de polimeros atraves de sondas fotocromaticas

Orientador : Teresa Dib Zambon AtvarsTese (doutorado) - Universidade Estadual de Campinas, Instituto de QuimicaDoutorad

Poly(vinyl Alcohol) And Poly(vinyl Pyrrolidone) Blended Films For Local Nitric Oxide Release.

The nitric oxide (NO) donor S-nitrosoglutathione (GSNO) was incorporated in solid polymeric films of poly(vinyl alcohol) (PVA), poly(vinyl pyrrolidone) (PVP) and blended PVA/PVP. These matrices were found to provide a great stabilization effect on the thermal decomposition of GSNO, leading to 8-16-fold reduction in the first-order rate constants of NO release, compared to aqueous GSNO solutions. PVA/PVP-GSNO released 90% of the NO supply, over a time period of 24h at 37 degrees C. Differential scanning calorimetry has confirmed the miscibility between the two polymeric components. Stress-strain analysis has shown an improvement of the mechanical property of PVA films in the PVA/PVP blend, which leads to an increase of 25% in the stress at break. Scanning electron microscopy has shown that the PVA/PVP-GSNO blend leads to a smooth coating of metallic surfaces. These properties, allied to the already known good biocompatibility of PVA and PVP, makes GSNO-containing PVA and PVA/PVP blend films good candidates for the local and controlled release of NO in target areas.253773-8

Filmes Poliméricos Sólidos De Poli(álcool Vinìlico) E Poli(álcool Vinìlico) - Poli (vinil Pirrolidona), Contendo S-nitrosotióis Doadores De óxido Nìtrico, Bem Como Os Métodos Para Sua Preparação

Resumo não disponível.BR0201167C08J5/18C08L29/04C08L29/04C08J5/18BR20020201167C08J5/18C08L29/04C08L29/04C08J5/1